mouse anti-calbindin antiserum  (Swant)

Journal: Biology of Sex Differences

Article Title: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis

doi: 10.1186/2042-6410-3-5

Figure Lengend Snippet: Ellipse and grid contours designed to quantify calbindin-immunoreactive (ir) cell number in and surrounding the calbindin-ir sexually dimorphic nucleus in the preoptic area (CALB-SDN) . The photographs in (A) and (B) are mirror-image duplications of a single image from a male mouse. (A) For CALB-SDN counts, an ellipsoid contour (solid line) was placed as indicated. A second calbindin-ir cell cluster was found dorsolateral to the CALB-SDN. These cells were determined to be the ventral extension of the bed nucleus of the stria terminalis (BNSTp) (dotted line) and were not included in CALB-SDN counts. (B) For counts of cells within and surrounding the CALB-SDN, a nine-block grid was placed as shown and counts were made within each block of the grid. Scale bar = 400 μm.

Article Snippet: Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X.

Techniques: Blocking Assay

Journal: Biology of Sex Differences

Article Title: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis

doi: 10.1186/2042-6410-3-5

Figure Lengend Snippet: Mean (± SEM) number of cells in the calbindin-immunoreactive sexually dimorphic nucleus in the preoptic area (CALB-SDN) of males, females, and females treated with a single injection of estradiol benzoate (EB) on the day of birth . N = 6 per group. Different letters above bars indicate significant differences between groups in post hoc tests.

Article Snippet: Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X.

Techniques: Injection

Journal: Biology of Sex Differences

Article Title: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis

doi: 10.1186/2042-6410-3-5

Figure Lengend Snippet: Grid analysis of calbindin-immunoreactive (ir) cells in and around the calbindin-ir sexually dimorphic nucleus in the preoptic area (CALB-SDN) in males, females, and females treated with a single injection of estradiol benzoate (EB) on the day of birth . Mean (± SEM) calbindin-ir cell number (A) within the total grid (sum of blocks 1 to 9); (B) in the central block of the grid only (which captures the majority of the CALB-SDN); and (C) in blocks 1 to 4 and 6 to 9 of the grid, reflecting calbindin-ir cells in regions surrounding the CALB-SDN. N = 6 per group. Different letters above bars indicate significant differences between groups in post hoc tests.

Article Snippet: Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X.

Techniques: Injection, Blocking Assay

Journal: Biology of Sex Differences

Article Title: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis

doi: 10.1186/2042-6410-3-5

Figure Lengend Snippet: Mean (± SEM) number of cells in the calbindin-immunoreactive sexually dimorphic nucleus in the preoptic area (CALB-SDN) of wild-type (WT) and Bax knockout (KO) males and females . There was a sex difference, but no effect of Bax genotype on CALB-SDN cell number. The number of animals per group is indicated at the base of each bar. Different letters above bars indicate significant differences between groups in post hoc tests.

Article Snippet: Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X.

Techniques: Knock-Out

Journal: Biology of Sex Differences

Article Title: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis

doi: 10.1186/2042-6410-3-5

Figure Lengend Snippet: Grid analysis of calbindin-immunoreactive (ir) cells in and around the calbindin-ir sexually dimorphic nucleus in the preoptic area (CALB-SDN) in wild-type and Bax knockout (KO) males and females . Mean (± SEM) calbindin-ir cell number (A) within the total grid (sum of blocks 1 to 9); (B) in the central block of the grid only (which captures the majority of the CALB-SDN); and (C) in blocks 1 to 4 and 6 to 9 of the grid, reflecting calbindin-ir cells in regions surrounding the CALB-SDN. The number of animals per group is indicated at the base of each bar. Different letters above bars indicate significant differences between groups in post hoc tests.

Article Snippet: Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X.

Techniques: Knock-Out, Blocking Assay

Journal: Biology of Sex Differences

Article Title: Effects of blocking developmental cell death on sexually dimorphic calbindin cell groups in the preoptic area and bed nucleus of the stria terminalis

doi: 10.1186/2042-6410-3-5

Figure Lengend Snippet: Mean (± SEM) number of calbindin-immunoreactive (ir) cells in the bed nucleus of the stria terminalis (BNSTp) of wild-type (WT) and Bax knockout (KO) males and females . There was a sex difference as well as a significant effect of Bax genotype. The number of animals per group is indicated at the base of each bar. Different letters above bars indicate significant differences between groups in post hoc tests.

Article Snippet: Sections were then exposed to primary antibody overnight (1:5,000 mouse monoclonal anti-calbindin-D28k antiserum (C9848; clone CB-955; Sigma-Aldrich)) in 2% NGS, and 0.3% Triton-X.

Techniques: Knock-Out

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Structural Correlates of Efficient GABAergic Transmission in the Basal Ganglia-Thalamus Pathway

doi: 10.1523/JNEUROSCI.5266-07.2008

Figure Lengend Snippet: Topography of nigrothalamic terminals and their relationship to calbindin immunostaining in the rat. A, Schematic labeling of five injection sites in the SNR shown at five coronal levels of the atlas of Paxinos and Watson (1998). B-G, Distribution of the dense core of nigrothalamic terminal fields in the thalamus, color coded according to the labeling in A. Scattered fibers are omitted for clarity. The outlines of the terminal fields are shown overlaid on calbindin-immunostained coronal sections of the thalamus sampled 300 μm apart. Note the dual terminal fields in the ventromedial and intralaminar nuclei and the long anteroposterior extent of the terminal fields. Caudal SNR injections result in more dense intralaminar fiber plexuses, whereas rostral injections label only few fibers in these nuclei. H, I, Light microscopic images of the VM double stained for BDA (black) and calbindin (brown) after injection of the tracer into SNR. The boxed area in H is shown enlarged in I. Nigrothalamic terminals (arrows in I) are restricted to the zone containing calbindin-immunoreactive cells (asterisks). J, Nigrothalamic terminals establish multiple contacts (arrows) on the soma of a calbindin-positive relay cell. I and J are multifocal images. Scalebars:B-G, 1 mm; H, 200 μm; I, 50 μm; J, 10 μm. AV, Anteroventral thalamic nucleus (n.); CL, centrolateral thalamic n.; F, nucleus of the fields of Forel; fr, fasciculus retroflexus; Hb, habenular n.; LD, laterodorsal thalamic n.; LH, lateral hypothalamic area; LP, lateral posterior thalamic n.; MD, mediodorsal thalamic n.; ml, medial lemniscus; mt, mammillothalamic tract; PaR, pararubral nucleus; PC, paracentral thalamic n.; PR, prerubral field; Po, posterior thalamic n. group; RMC, red nucleus, magnocellular part; RPC, red nucleus, parvicellular part; sm, stria medullaris of thalamus; SNC, substantia nigra, compact part; Sub, submedius thalamic n.; VA, ventral anterior thalamic n.; VL, ventrolateral thalamic n.; VTA, ventral tegmental area; ZID, zona incerta dorsal part; ZIV, zona incerta ventral part.

Article Snippet: After visualizing the tracer with DABNi, sections containing anterogradely labeled terminals were incubated with mouse anti-calbindin antiserum (1:4000, overnight; Swant, Bellinzona, Switzerland).

Techniques: Immunostaining, Labeling, Injection, Staining